Bryan Brothers Upset In First Round Of US Open

Florian Mayer of Germany (L) and Ivo Karlovic of Croatia celebrate a point against Bob Bryan of the United States and Mike Bryan of the United States during Day Three of the 2011 US Open at the USTA Billie Jean King National Tennis Center on August 31, 2011. (credit: Matthew Stockman/Getty Images)

Florian Mayer of Germany (L) and Ivo Karlovic of Croatia celebrate a point against Bob Bryan of the United States and Mike Bryan of the United States during Day Three of the 2011 US Open at the USTA Billie Jean King National Tennis Center on August 31, 2011. (credit: Matthew Stockman/Getty Images)

NEW YORK (AP) — The top-ranked Bryan brothers lost a first-round doubles match at a Grand Slam tournament for the first time since 2001, making an early exit from the U.S. Open on Wednesday.

Unseeded Ivo Karlovic and Frank Moser beat the American twins 6-4, 2-6, 6-2. Bob and Mike Bryan had won three of the last four major titles, tying the Open era record with their 11th Grand Slam championship earlier this summer at Wimbledon.

But they struggled against the 6-foot-10 Karlovic’s powerful serve.

Copyright 2011 The Associated Press.