Elliot Morales, 33, pleaded not guilty to murder as a hate crime in the fatal shooting of 32-year-old Mark Carson.
Gornell Roman was charged Wednesday with assault and aggravated harassment, both as hate crimes, police said.
The incident happened around 5:20 a.m. Tuesday. Police said a gay couple, ages 41 and 42, were walking on Broadway near Prince Street when two men began yelling anti-gay slurs.
A community is rallying against hate, and police are increasing patrols in some neighborhoods – in the wake of five anti-gay attacks in the past two weeks.